With our PhenoStart™ Lite process we can evaluate protein expression in as little as 6 weeks. If this is positive, we move into a full feasibility PhenoStart™ for a further 10 weeks. We will establish if we can make the protein effectively and what titre and broad quality we can aim for.
Positive results from the PhenoStart™ stage could lead into a PhenoDev™ project which can take up to 35 weeks depending on the protein and complexity. We are aiming to produce the optimal industrial production strain to go into fermenters. At the end of the service, you will be provided with an animal free R&D cell bank of 3-6 strains and associated plasmids optimised strains for use in your further downstream process development either in-house or with a CDMO of your choice. We can provide additional technology transfer to a CDMO, or you can progress to our PhenoFull™ service.
PhenoFull™ can be used to develop your specific process for low-cost protein production. You will get an R&D scale process description for subsequent scale-up, validated for yield in industrially relevant fermentation conditions using animal-free media and with cell separation, purification and quality attributes designed for cGMP manufacturing. Final formulations could also be generated for stability testing and some pre-clinical work. The nature of this ‘campaign’ style of work means that it is difficult to define exact timelines, but typically this could range from 50-75 weeks.